牛卵泡TEDDM1表达特点及其功能分析

doi:10.11843/j.issn.0366-6964.2019.06.009

Abstract

Abstract: The aim of this study was to identify molecular characteristic and three-dimensional structure of TEDDM1 in bovine follicle, its function was analyzed by combining expression characteristic of TEDDM1 in different physiology states of follicles. DF and SF in first follicular development wave during estrous cycle were collected, GCs were isolated, total RNA was extracted and the reverse transcription was performed by RT-PCR, the specific primers were designed to amplify TEDDM1 and the sequence was sequenced, bioinformatics method was used to analyze CDS region sequence structure; Using RPLP0 as reference gene, qRT-PCR was performed to detect the experssion of TEDDM1 in DF and SF; The rabbit anti-TEDDM1 antibody was used to dectet expression level and localization of TEDDM1 in bovine follicles. The results showed that full length of TEDDM1 CDS region was 903 bp, encoding 300 amino acids, contained 7 parallel alpha helical structures across the cell membrane, which was a typical G protein-coupled receptor, sequence of TEDDM1 had the highest similarity with Bison (99.4%); The functional domain analysis showed that DUF716 existed in TEDDM1. The results of qRT-PCR analysis showed that expression level of TEDDM1 mRNA in SF was significantly higher than that in DF (P<0.05); The immunohistochemical analysis showed that TEDDM1 was expressed in GCs and membrane cells layer of DF and SF, and specific color intensity result showed that expression of TEDDM1 in SF GCs and membrane cells was higher than that in DF. The study provides a basis for further study on regulatory effect of TEDDM1 in bovine follicular development, signal transduction and hormone regulation, and lays a foundation for further research on mechanism of bovine follicular development.

CLC Number:

S823.3

ZHU Zhiwei, HAO Qingling, HOU Shuning, JING Jiongjie, ZHAO Chengping, Lü Lihua, LI Pengfei. Expression Characteristic and Function Analysis of TEDDM1 in Bovine Follicle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1189-1197.

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Expression Characteristic and Function Analysis of TEDDM1 in Bovine Follicle

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